Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS.
نویسندگان
چکیده
A simple, cost-effective and high throughput method was developed for multiplexed kinase activity assay based on the multiplex isotope labeling of designed substrate peptides. This strategy was successfully applied to monitor the time-dependent consumption of substrates and generation of products in the single and multiple substrate systems.
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ورودعنوان ژورنال:
- Chemical communications
دوره 50 90 شماره
صفحات -
تاریخ انتشار 2014